Scientific journal
Научное обозрение. Медицинские науки
ISSN 2500-0780
ПИ №ФС77-57452

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IONIC MECHANISMS OF CARBON MONOXIDE ON CONTRACTILE ACTIVITY OF VASCULAR SMOOTH MUSCLES

Zheludeva A.S. 1
1 Sibirian State Medical University
Using methods of mechanography and radionuclide techniques we investigated the mechanism of carbon monoxide (CO) action on the contractile responses of vascular smooth muscle cells (SGMK). It was shown that the donor CO - CORM-2 in concentrations of 10-1000 µМ in the case of high potassium contractions, and in concentrations of 1 µМ and above in the case of phenilefrine-induced contractions (10 µМ) of smooth muscles caused a dose-dependent relaxation of vascular segments. Inhibitors of NO-synthase and soluble guanylate cyclase (GC) weakened CO - induced relaxation of the segments. CORM-2 significantly decreased nicardipine-sensitive input of 45Са2+ in freshly isolated aortic smooth muscle cells. Dose-dependent relaxation of the CO is caused by opening of voltage-dependent and Са2+activated K+ channels and by decreasing of Са2+entry through L-type of voltage-dependent Са2+ channels of vascular smooth muscle cells. For the first time we established the involvement of L-type voltage-dependent Са2+ channels in mechanisms of relaxing action of СО.