Scientific journal
Научное обозрение. Медицинские науки
ISSN 2500-0780
ПИ №ФС77-57452

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THE ROLE OF NITRIC OXIDE IN THE MECHANISM OF ACTION OF CARBON MONOXIDE ON THE CONTRACTILE ACTIVITY OF SMOOTH MUSCLE CELLS FROM THE RAT AORTA

Baskakov M.B. 1, 2 Zheludeva A.S. 1 Gusakova S.V. 1 Smagly L.V. 1 Kovalev I.V. 1 Birulina J.G. 1 Aleinik A.N. 2 Medvedev M.A. 1 Orlov S.N. 3
1 Sibirian State Medical University
2 National Research Tomsk Polytechnic University
3 Research Center
The effect of carbon monoxide (CO) on contractile reactions of smooth muscle cells (SMC) caused by depolarization (30mM KCl) and phenylephrine (PE) has been investigated by the mechanographical method. It is shown that the donor CO - CORM-2, in experiments with of high potassium contraction in concentrations of 10-1000 µМ, and in cases phenilefrine-induced contractions (10 µМ) of SMC, of 1 µМ and above, caused a dose-dependent relaxation of vascular segments. Inhibition of NO-synthase and soluble guanylate cyclase (GC) weakened CO - induced relaxation of the segments. With blocking potassium channels tetraethylammonium chloride and 4-aminopyridine relaxing effect СORM-2 on vascular segments virtually eliminated. These results may be the evidence of cooperation in the implementation of gazotransmitters gasotransmitters relaxating effect of CO on the level of soluble guanylate cyclase and potassium channels plasmalemma of SMC.