Scientific journal
Научное обозрение. Медицинские науки
ISSN 2500-0780
ПИ №ФС77-57452

USING OF PCR IN THE DIAGNOSTICS OF INFLAMMATORY DISEASES OF THE REPRODUCTIVE TRACT

Potaturkina-Nesterova N.I. 1 Nemova I.S. 2
1  Tolyatti State University
2  Ulyanovsk State University
Currently, PCR is a highly sensitive methods for the diagnosis of infectious diseases, which can detect single bacterial cells. It is shown that the diagnostic and economic efficiency of pathogens detection is enhanced by simultaneous PCR detection of the most significant pathogens in a mixture of informative substrates. The purpose of this work is comparing PCR efficiency with various means of Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum DNA isolation among the patients with urogenital infections (UI). It was found out that in case of DNA isolation using sorbent, the maximum amplification product was observed in comparison with phenol-chloroform precipitation method of extraction. It testifies to more complete DNA extraction from bacteria. Such a PCR method was more effective while diagnostics of UI mycoplasma etiology.