Scientific journal
Научное обозрение. Медицинские науки
ISSN 2500-0780
ПИ №ФС77-57452

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FLUORESCENT INDICATORS OF LIVING TUMOR CELLS OF SQUAMOUS EPITHELIUM BEFORE AND AFTER RADIOTHERAPY IN PATIENTS WITH SQUAMOUS CELL CARCINOMA OF THE ORAL CAVITY

Rosenko L.J. 1 Tarnopolskaya O.V. 1 Shikhlyarova A.I. 1 Nepomnyashaya E.M. 1 Krochmal J.N. 1
1 Federal State Budget Institution “Rostov Research Oncological Institute” Ministry of Health of Russian Federation. Rostov-on-Don
The fluorescence intensity of vital dyes DCM (4 - (n-dimethylaminostyryl) -1 - methylpyridinium), ANS and doxorubicin (DR) associated with tumor cells of squamous cell carcinoma of the oral cavity human leukocytes inhabiting the tumor before and after chemoradiotherapy by fluorescence microscopy have been examined. Chemotherapy with cisplatin was performed under ultrasound exposure. For cells stained DSM obtained statistically significant differences in mean fluorescence intensity of tumor cells in groups of patients before and after treatment (p = 0.038) reduction in the intensity of 1.7. This suggests that the performance of DSM fluorochrome associated with tumor cells reflect the results of treatment may be a criterion for the presence of cisplatin in cells. For cells in the color of doxorubicin and ANS from patients before and after treatment, no significant difference in intensity is not revealed. In coloring ANS well visualized live and dead cells, as visualized in color DR preferential accumulation this dye in the cell nuclei.