Scientific journal
Научное обозрение. Медицинские науки
ISSN 2500-0780
ПИ №ФС77-57452

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THE CIRCADIAN RHYTHMS OF APOPTOSIS SPERMATOGENIC CELLS IN INTACT WHITE RATS AND ITS BREACH AFTER PINEALECTOMY

Slesareva E.V. 1 Slesarev S.M. 1 Arav V.I. 1 Lyapeykova O.V. 1 Gulchin A.A. 1
1 Ulyanovsk state University
It was determined the enzyme activity - inducers of apoptosis in spermatogenic cells in intact male white rats and white rats after pinealectomy by immunohistochemistry. Determination of enzyme was carried out in the dark (1 h) and light (13 h) time of day for two days. The pinealectomy was performed by the authors’ methodology. The enzyme activity was evaluated after 40 days after the removal of the pineal gland, when the influence of the fact of surgery had become insignificant. It was identified the procaspase 3 and PARP-1 (p116/25) activity as the enzymes involved in DNA repair and apoptosis activation using immunohistochemical staining of paraffin sections of testes. In intact animals was revealed the circadian rhythm of activity of the studied enzymes with higher levels of activity in the night time, which correlates with the dynamics of proliferation of spermatogonia. The lack of epiphyseal control for 40 days resulted to a significant increase of the level of the these enzymes in maturing germ cells and the disappearance of the circadian rhythm of their dynamics. This fact indicates the growth of damages in the DNA structure of spermatogenic cells after pinealectomy.