Scientific journal
Научное обозрение. Медицинские науки
ISSN 2500-0780
ПИ №ФС77-57452

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ERYTHROPOIETIN INFLUENCE ON IMMUNOLOGICAL STATUS INDICIES IN EXPERIMENTAL CHRONIC RENAL FAILURE

Ageev J.I. 1 Osikov M.V. 1 Telesheva L.F. 1 Fedosov A.A. 1
1 South Ural State Medical University of Health Ministry of Russia
Discovery of EPO receptors on nonerythroid tissues cells at the beginning of XXI century enabled to investigate EPO non-hematopoietic functions. In 2010 EPO receptors were revealed on T and B-lymphocytes, monocytes, suggesting glycoprotein immunotropic effects. Hypothesis testing is convenient to perform in experimental chronic renal failure (CRF), when endogenous EPO production is significantly reduced. Aim of investigation: EPO efinfluence on innate and adaptive immunity in experimental CRF. Of 115 white nonlinear rats: group I comprised controls, sham-operated animals; group II animals with CRF; group III animals with CRF with EPO administration. CRF was modeled by two-stage operative resection of 5/6 renal tissue. EPO in preparation “Epokrin” (epoetinalfa, St. Petersburg) was used at 100 IU/kg dose during 9 days. Investigation was performed on day 30. Immune status was evaluated by leukocytes amount, leucocyte formula, leukocytes absorbability using monodisperse polystyrene latex particles, phagocytes oxygen-dependent metabolism in spontaneous and induced nitrobluetetrazolium test. T-helper-1 or T-helper-2-dependent immune response was evaluated by antibody producing cells number in the rats’ spleen, which were immunized with allogeneic erythrocytes. In experimental CRF absolute amount of phagocytic cells (segmented neutrophils, monocytes) increases in peripheral blood, lymphocytes amount reduces. Innate immunity activation is manifested by increased absorbency and oxygen-dependent metabolism of phagocytic cells. In assessing adaptive immunity, inhibition of cellular (T-helper-1-dependent) and humoral (T-helper-2-dependent) immune responses was noted. EPO in a total dose of 900 IU/kg results in CRF experimental quantitative restoration of leukocytes, phagocytic activity and oxygen-dependent metabolism of phagocytes, indicators of T-helper-1 or T-helper-2-dependent immune response.